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Ana Luísa Matos was born in Porto, Portugal. After earning a bachelor degree in Graphic Design from ESAD College of Art and Design in Matosinhos, Portugal she worked as a freelance designer, focusing mainly on editorial projects. In 2010 she studied at the Portuguese Institute of Photography and after graduating worked as a photojournalist. In 2014 she was based in Rosario, Argentina working for several NGOs, including Techo and Rosario Food Bank.

She is a graduate of the Visual Journalism and Documentary Photography at the International Center of Photography in New York.